DNA cytosine methylation is the addition of a methyl group to a cytosine in the DNA. It impacts transcription and therefore plays a major role in several vital processes. In mammals, DNA cytosine methylation predominantly occurs in CG sequence contexts. In plants, in addition to the CG context, the CHG and CHH contexts are common as well.
Various tools have been introduced to facilitate the analysis of DNA cytosine methylation data. Usually, they focus on a small part of the workflow, which still leaves users with a considerable amount of work to evaluate appropriate tools, transform intermediate output, and finally generate publication-ready figures. Additionally, many tools are limited in terms of the input data, only providing support for certain species and/or specific library preparation methods.
Here we introduce Methylator, a user-friendly tool for a full DNA cytosine methylation analysis, with an easy-to-use interface, facilitated reproducibility and interactive visualizations of results.
Galaxy is an open-source, web-based platform for accessible, reproducible, and transparent computational biomedical research. It provides a user-friendly interface for executing complex bioinformatics tools and workflows without requiring programming expertise. Galaxy enables researchers to upload their data, run analyses using a vast array of tools, and share their entire analytical workflow with others. This approach not only facilitates collaboration but also enhances the reproducibility of scientific findings.